{"id":398,"date":"2023-08-15T21:38:57","date_gmt":"2023-08-15T19:38:57","guid":{"rendered":"http:\/\/sensorarrays.com.pl\/?p=398"},"modified":"2025-05-20T14:07:10","modified_gmt":"2025-05-20T12:07:10","slug":"karthikas-article-in-lab-on-a-chip","status":"publish","type":"post","link":"https:\/\/sensorarrays.com.pl\/?p=398","title":{"rendered":"Karthika’s article in Lab on a Chip"},"content":{"rendered":"\n

Karthika’s publication, “Fabrication of ITO microelectrodes and electrode arrays using low-cost CO2<\/sub> laser plotter<\/em>,” has been recently accepted in Lab on a Chip<\/a>. The article shows a cost-effective method based on CO2<\/sub> laser plotter irradiation for preparing ITO microelectrodes. The work was supposed to be a side project but grew and grew till it became a collaboration between our and three more IPC PAS groups. <\/p>\n\n\n\n

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Our CO2<\/sub> laser, generally used to prepare paper-based electrodes, can also easily ablate ITO layer on glass. Electrodes of different sizes and shapes can be prepared in this manner. At first, rectangular-shaped electrodes were made using direct laser ablation; the smallest sizes optimized were 25, 50, and 100 \u00b5m wide. <\/p>\n<\/div>\n<\/div>\n\n\n\n

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The results of the narrowest electrodes with a nominal width of 25 \u00b5m were not so reproducible; they were significantly wider (39\u00b13.5 \u00b5m) than the nominal width (25 \u00b5m). However, the larger electrodes performed very well.<\/p>\n\n\n\n

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In a second technique, we engraved patterns onto scotch tape that covered the ITO\/glass plates using the CO2<\/sub> laser. The conducting layer, except the electrodes, was exposed and removed using a solution of FeCl3<\/sub> and HCl. The cut tape was utilized as a mask to shield the ITO layer on the electrode. This in-situ stencil fabrication followed by etching was the best practice for larger circular-shaped electrodes (diameter 1.75 mm, 2.41 mm2<\/sub> of geometrical surface area), which were used for impedance evaluation of the state of HepG2 and Hela cell cultures. <\/p>\n<\/div>\n<\/div>\n\n\n\n

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Both these methods are efficient and inexpensive means of electrode fabrication.<\/p>\n\n\n\n

To show that they can be used in resource limited settings, we even went to a local printing shop that had the same equipment and made few batches of electrodes outside the lab!<\/p>\n\n\n\n

Even though their reproducibility was a bit lower, they functioned fine. This helped us demonstrate that this technique is practical and can be used even by researchers who don’t have a laser plotter.<\/p>\n<\/div>\n\n\n\n

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Findings from the NCBR LIDER\/38\/0138\/L-9\/17\/NCBR\/2018 project<\/em>.<\/p>\n","protected":false},"excerpt":{"rendered":"

Karthika’s publication, “Fabrication of ITO microelectrodes and electrode arrays using low-cost CO2 laser plotter,” has been recently accepted in Lab on a Chip. The article shows a cost-effective method based on CO2 laser plotter irradiation for preparing ITO microelectrodes. The work was supposed to be a side project but grew and grew till it became…<\/p>\n","protected":false},"author":1,"featured_media":399,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3,26,1],"tags":[5],"class_list":["post-398","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-general-news","category-publications","category-uncategorized","tag-success"],"_links":{"self":[{"href":"https:\/\/sensorarrays.com.pl\/index.php?rest_route=\/wp\/v2\/posts\/398","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sensorarrays.com.pl\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sensorarrays.com.pl\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sensorarrays.com.pl\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sensorarrays.com.pl\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=398"}],"version-history":[{"count":6,"href":"https:\/\/sensorarrays.com.pl\/index.php?rest_route=\/wp\/v2\/posts\/398\/revisions"}],"predecessor-version":[{"id":1011,"href":"https:\/\/sensorarrays.com.pl\/index.php?rest_route=\/wp\/v2\/posts\/398\/revisions\/1011"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sensorarrays.com.pl\/index.php?rest_route=\/wp\/v2\/media\/399"}],"wp:attachment":[{"href":"https:\/\/sensorarrays.com.pl\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=398"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sensorarrays.com.pl\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=398"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sensorarrays.com.pl\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=398"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}